Jordan Henderson was the main worry after he was withdrawn towards the end of the victory over Sweden due to a tight hamstring, but he is believed to have reacted well to treatment and should be fit to take up his place in midfield.
The Liverpool midfielder has been a mainstay of the England side so far in the tournament, starting four out of England's five games to date, missing only the defeat to Belgium in which Gareth Suothgate fielded a much-changed side.
Jamie Vardy also trained as normal after he tweaked a groin against Colombia, a niggle that prevented him from taking a penalty in the shootout.
The news means Southgate could opt to name an unchanged side against Croatia at the Luzhniki as England look to reach just their second World Cup final in history.