The Manchester United manager was sent to the stands in the first half of the home draw against West Ham for kicking a water bottle in frustration, after Paul Pogba was harshly booked for a dive.
The Portuguese manager admitted the charge of improper conduct and received the one-match ban and fine.
It was the second time that the former Chelsea boss has been sent to the stands this season, as he was also sent off against Burnley in the 0-0 draw at Old Trafford.
Mourinho will still be able to speak to the players in the dressing room before the game, at half-time and after the game, and will also be able to communicate with members of staff on the United bench during the game.