The game Nations League game between England and Croatia will be played behind closed doors due to a swastika symbol was being marked on their pitch before a Euro 2016 qualifier in 2015.
Bar the media and officials, the stadium will be virtually empty due to UEFA sanctions, TV cameras and microphones are therefore likely to pick up players voices during the game.
Despite Southgate's words of warning, he accepts it may be difficult to completely eradicate the expletives from his players in the heat of battle.
"I think it's for the television companies to decide where they put their microphones," he told 'Daily Mail'.
"So it's something we can't prepare for, other than that we train in the same sort of environment every day of the week", he added.
Up to 500 England fans are due to be in Rijeka despite the supporter ban - there are a number of vantage points around the stadium that would allow fans to watch the game.