The Rainbow Laces campaigned has been running for some years now, and sees players don multicoloured laces to show their support for the LGBT cause.
Between November 24 and December 1, clubs in the EFL will choose a match to show their committment to the cause. Captain's armbands, laces and adverts in programmes will all have a rainbow theme.
The EFL logo will also be changed to a rainbow coloured version, and a short series of films have been made to help share the experiences of LGBT fans and others active in the game.
Premier League matches, for their part, will feature a Rainbow Laces pitch flag, ball plinth, handshake board and substitutes board.
Shaun Harvey, EFL Chief Executive said: “The EFL is once again fully supporting Stonewall’s Rainbow Laces campaign, with all 72 Clubs coming together to raise awareness and visibility of an important issue.
"The EFL continues to be committed to providing a match day experience that is safe and welcoming to all supporters across all of our competitions."
"Stonewall is an excellent partner for us and we value their ongoing work to support the league and our clubs in promoting diversity across the sport," added Premier League executive director Bill Bush said.
"Our clubs do fantastic work in this area to reinforce the message that football is for everyone. We are proud to be playing our part and to use our reach to celebrate the Rainbow Laces campaign."