Inter defender Bastoni had been struggling for fitness and will not travel to Belfast for Monday's game, with Calabria experiencing a calf problem on Saturday and Biraghi dropping out for personal reasons.
To bolster his defensive options, with veteran centre-back Giorgio Chiellini having left the camp ahead of Friday's 1-1 draw with Switzerland, Roberto Mancini called up Davide Zappacosta.
Italy sit top of Group C on goal difference so only need to match Switzerland's result at home to Bulgaria when they take on Northern Ireland in order to clinch top spot.
A draw would have been enough had Jorginho converted a 90th-minute penalty against Switzerland on Friday, but the Chelsea midfielder missed his third successive spot-kick (including shoot-outs) for the Azzurri.
Italy have won seven of their 10 matches against Northern Ireland, who have been scoreless in the past six and secured their only victory in a World Cup qualifier back in January 1958.
However, the last meeting between the pair in Northern Ireland was a goalless draw at Windsor Park in October 2010.
Standout matches today