Italy forward Insigne looked a doubt for the second leg of the last 16 tie, in which the two sides are locked at 1-1, after picking up the knock during Napoli's final match of the Serie A season against Lazio a week ago. However he takes the field in Napoli's front three alongside Dries Mertens and Jose Callejon.
Barca coach Quique Setien meanwhile plumped for a predictable line-up thanks to injuries and suspensions that left him thin in midfield. The Spanish coach is without the not-yet match fit Ousmane Dembele and Sergio Busquets and Arturo Vidal, who are both suspended. Meanwhile Juventus-bound Arthur is not in the squad following a row with the club.
That means that Sergi Roberto, Ivan Rakitic and Frenkie de Jong line up in a three-man midfield behind Lionel Messi, Luis Suarez and Antoine Griezmann.
Teams:
Barcelona (ESP) 4-3-3
Marc-Andre Ter Stegen; Nelson Semedo, Gerard Pique, Clement Lenglet, Jordi Alba; Sergi Roberto, Ivan Rakitic, Frenkie de Jong; Lionel Messi, Luis Suarez, Antoine Griezmann
Coach: Quique Setien (ESP)
Napoli (ITA) 4-3-3
David Ospina; Giovanni Di Lorenzo, Kostas Manolas, Kalidou Koulibaly, Mario Rui; Fabian Ruiz, Diego Demme, Piotr Zielinski; Jose Callejon, Dries Mertens, Lorenzo Insigne
Coach: Gennaro Gattuso (ITA)
Referee: Cuneyt Cakir (TUR)