Paraguay have not defeated Uruguay at the Copa America since 1947, but La Albirroja have momentum entering Monday's final group-stage game in Rio.
While Uruguay have laboured to find their form in the tournament, Paraguay have taken six points from the first three Group A matches.
They defeated Chile 2-0 on Thursday to confirm their place in the quarter-finals, three days after Uruguay needed an own goal by Arturo Vidal to salvage a 1-1 draw with La Roja.
Coupled with a tournament-opening defeat of Bolivia, Paraguay will be feeling confident about their chances to earn a long-awaited victory.
Head coach Eduardo Berizzo said he expects the game to unfold along similar lines as the sides' scoreless draw in World Cup qualifying in Montevideo earlier this month.
Entrenamiento de hoy en Río de Janeiro.
Este lunes, Paraguay vs Uruguay.#ElSueñoQueNosUne