Sevilla face on Tuesday Real Betis in their first derby this season.
Adil Rami left pitch the last game earlier due to calf problems. Daniel Carrico will play neither as he is suffering a thigh injury.
Jorge Sampaoli said: "Today we lost two and we have to see which ones [defenders] are able to compete," the Argentine told reporters. As for Rami and Carrico, both are complicated, the diagnosis we do not know yet. They have to be evaluated in the coming hours but I do not think they can play on Tuesday.