Portugal: The reigning European champions have no injury concerns going in to this crucial match, and will rely on the familiar figure of their captain, Cristiano Ronaldo, to help inspire them to victory.
Portugal also boast the attacking talents of Bernado Silva to complement the threat of their main man, while veterans Pepe and Bruno Alves are a proven paring that will prove a tough obstacle for the Spanish attacking line.
Spain: The 2010 World Champions faced huge turmoil in the run-up to this game, following the much talked-about sacking of their manager Julen Lopetegui on Wednesday, but Fernandoo Hierro's team will look to put that behind them in this vital match.
Spanish captain Sergio Ramos and his central defensive partner Gerard Pique know the threat Cristiano Ronaldo poses better than any pairing at the tournament, while right-back Daniel Carvajal is a doubt having picked up a hamstring injury.
An interesting choice needs to be made up front by Fernando Hierro, who could choose the mercurial Diego Costa or opt for in-form Iago Aspas.
Portugal Manager Fernando Santos on what his team will face:
“There will be two on-field teams that will want to win the game. The most important thing is the clash between the two teams. It has everything to be a great game.
“We are prepared for a tricky game but we are confident that we are capable.”
Spanish captain Sergio Ramos on his Real Madrid teammate Cristiano Ronaldo:
"We know how important Cris is, and that he finished the season in great form which we are constatly aware of. But Portugal isn't only Cris, they have a really strong squad, which is very competitive, and tomorrow's game will be an exciting one. Obviously tomorrow, I won't be wishing the best for Cris."
Did you know?
The last time Portugal beat Spain in an international tournament was at Euro 2004, where a teenage Cristiano Ronaldo was playing in his first major tournament.
Prediction: 1-2
Possible lineups:
Portugal: Rui Patricio, Cédric, Pepe, Bruno Alves, Guerreiro, B.Silva, Moutinho, Carvalho, Fernandes, Gonçales Guedes, C.Ronaldo
Spain: De Gea, Odriozola, Pique, Ramos, Alba; Alcantara, Busquets, Iniesta; Isco, Costa, D. Silva