Dortmund currently trail in the table by four points, a deficit which could stretch to seven if Bayern win at strugglers Augsburg on Friday.
This will be Dortmund's sixth consecutive match without Haaland and hopes are fading that the Norwegian will return before their league showdown at home to Bayern in two weeks.
Dortmund's form without Haaland has been erratic, culminating in a 2-1 defeat at RB Leipzig before the international break.
Club captain Marco Reus will lead Dortmund's attack alongside Dutch striker DonyellÂ Malen knowing the hosts can ill afford another slip-up against Stuttgart if they want to challenge Bayern.
"We have to be incredibly consistent and be ready when they (Bayern) falter, but they hardly ever do," Reus told Sport1.de.
Raphael Guerreiro, Emre Can and Mahmoud Dahoud could all return after recovering from injuries.
Dortmund want to exorcise memories of the 5-1 thumping Stuttgart handed them when the teams met at Signal Iduna Park last December.
The freak result saw Dortmund sack former coach Lucien Favre as Stuttgart's Congolese striker Silas Katompa Mvumpa netted twice in the rout.
It is also a tune-up for Wednesday's Champions League match at Sporting Lisbon, where Dortmund must win to stay on course for the last 16.
Leipzig are without injured striker Yussuf Poulsen for their match at Hoffenheim on Saturday.
"That was the worst news for us during the international break," said Leipzig coach Jesse Marsch after Poulsen tore a calf muscle while away with the Danish team.
In the capital, tensions will be high for Saturday's derby when Union Berlin host Hertha.
A capacity crowd of 22,000 is expected despite surging numbers of Covid-19 in Germany and only fully vaccinated or recovered supporters can attend.
"It's great to have a full stadium, the atmosphere will be terrific, but you have it at the back of your mind that the numbers are going up again rapidly at the moment," admitted Hertha defender Niklas Stark.
This is only the fifth time the neighbouring clubs have met in Germany's top flight since Union reached the Bundesliga in 2019.
It finished in a 1-1 draw when the clubs last played in east Berlin last April.
Player to watch: Genki Haraguchi
Union's Japan midfielder faces former club Hertha Berlin on the back of great form after switching from the wing to an attacking midfield role.
"He's one of the winners of the season so far," said Union CEO Oliver Ruhnert.
After struggling on the wing at Hertha, Haraguchi regained his confidence in the second tier at Hanover and is thriving in a more central position at Union, who he joined in July.
His box-to-box energy and slick passes created four goals in wins over Moenchengladbach and Wolfsburg, a home defeat to Bayern Munich and draw with Cologne.
During the international break, he briefly came on in Japan's wins over Oman and Vietnam.
"He's a bit like the Duracell bunny - he always gives his all," added Ruhnert.
Standout matches today