Videos on social media showed fans in the Chivas home section of the stadium making 'monkey chants' in the direction of Club America winger Julian Quinones, who is black. Club America had made a formal complaint to CONCACAF, the governing body for football in North and Central America and the Caribbean.
"CONCACAF is extremely concerned about the video circulating on social media... the Confederation will conduct a full investigation of all evidence, including obtaining statements from both clubs and match officials," the governing body said in a statement.
"CONCACAF condemns any act of racism in our sport and society. Passionate support must never include discrimination. What is wrong is wrong."
FIFA, the sport's global governing body, has called for "zero tolerance" in cases of racism.