The Catalan side travel to Wembley on Wednesday in the hopes of beating a Tottenham side that lost 2-1 to Inter Milan in its first Champions League match.
According to Spanish publication 'Sport', Valverde will make a switch from his usual 4-3-3 to a 4-4-2, suggesting Ousmane Dembele could be dropped.
Valverde is expected to leave the French winger on the substitutes bench to reinforce Barcelona's strength in midfield and defence for the European clash.
Meanwhile, £45million summer signing Malcom is set to be axed from the squad once again.
The former Bordeaux winger missed out during Barcelona's 1-1 draw against Athletic Bilbao on Saturday afternoon in La Liga.