Northern South
End
Non League Div One
Bedworth United
2-2
Stocksbridge Park Steels
27 AUG 2016
End
Non League Div One
Carlton Town
1-3
Rushden & Diamonds
27 AUG 2016
End
Non League Div One
Chasetown
0-0
Belper Town FC
27 AUG 2016
End
Non League Div One
Gresley
1-1
Leek Town
27 AUG 2016
End
Non League Div One
Kidsgrove Athletic
0-1
Rugby Town
27 AUG 2016
End
Non League Div One
Lincoln United FC
1-2
Romulus
27 AUG 2016
End
Non League Div One
Market Drayton Town
0-3
Loughborough Dynamo FC
27 AUG 2016
End
Non League Div One
Newcastle Town
3-1
Basford United
27 AUG 2016
End
Non League Div One
Northwich Victoria
1-3
Spalding United
27 AUG 2016
End
Non League Div One
Sheffield FC
0-1
Witton Albion
27 AUG 2016
End
Non League Div One
Stamford
1-1
Shaw Lane Aquaforce
27 AUG 2016